Large-Format 2-D Polyacrylamide Gel Electrophoresis ...
Therefore, proteins vary widely in size and have either positive, negative, or zero net charge, depending on the pH of their surroundings. The original two-dimensional gel electrophoresis format was developed almost 30 years ago to exploit this variation in protein charge and size for separation purposes. The isoelectric point of a protein (pI) is the pH at which it has a net zero charge, which, for the majority of …
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