Just fill in the form below, click submit, you will get the price list, and we will contact you within one working day. Please also feel free to contact us via email or phone. (* is required).

cost of 5 polyacrylamide gel recipe

  • SDS Polyacrylamide Gel Electrophoresis

    2011-7-14 · 50 ml 20% SDS 7.5 ml 20% SDS dH 2 O to 2 liters dH 2 O to 1.5 liters Laemmli Sample Preparation Buffer: DTT: 123.4 mg Glycerol or 50% sucrose 4 ml 0.2 M Tris, pH 8.0/20 mM EDTA 1 ml 20 mg% pyronine Y 1 ml 20% SDS 1 ml dH 2 O 1 ml This comes out to: 2.5% SDS, 100 mM DTT, 25 mM Tris, 2.5 mM EDTA. Can be diluted with twice the volume of sample (ie. 3-fold dilution)

    Get Price
  • A Guide to Polyacrylamide Gel Electrophoresis and

    2018-3-5 · into two sections (a large-pore stacking gel on top of a small-pore resolving gel, Figure 2.2) and different buffers in the gels and electrode solutions (Wheeler et al. 2004) In gel electrophoresis, proteins do not all enter the gel matrix at the same time. Samples are loaded into wells, and the proteins that are closer to the gel

    Get Price
  • DNA Polyacrylamide Gel Electrophoresis

    2013-11-4 · acrylamide gel solution to fill the gel mold completely. Make sure that no acrylamide solution is leaking from the gel mold. 6. Allow the acrylamide to polymerize for 30-60 minutes at room temperature. 7. After polymerization is complete, surround the comb and the top of the gel with paper towels that have been soaked in 1x TBE. Then seal the

    Get Price
  • Denaturing Polyacrylamide Gel Electrophoresis

    2019-2-5 · polyacrylamide around gel. 19. Hold two pieces of dry 46 × 57–cm blotting paper together as one piece. Beginning at one end of gel and working slowly towards the other, lay paper on top of gel. Take care to prevent air bubbles from forming between paper and gel. 20. Peel blotting paper up; gel should come off plate with it. Gradually curl paper and

    Get Price
  • Non-denaturing PAGE - Thermo Fisher Scientific

    2018-8-21 · 1. For a nondenaturing 5% polyacrylamide gel solution of 40 ml, mix the following: 10X TBE Buffer 4 ml 20% acrylamide/bisacrylamide 10 ml Deionized water 26 ml 2. Caution: acrylamide is a neurotoxin; always wear gloves, safety glasses, and a surgical mask when working with acrylamide powder. 3. Vigorously agitate the solution for 1 min by

    Get Price
  • SDS-PAGE Gel Recipes | Proteintech Group

    Western Blot ppt. Western Blot Video Protocol. In order to target proteins with MWs between 20 and 200 kDa, you will need to create a conventional SDS-PAGE gel using the recipes shown below. The percentage of gel you require corresponds with the MW of your target protein. Recipe 1.

    Get Price
  • Hand Casting Polyacrylamide Gels | LSR | Bio-Rad

    The glass plates must be clean and free of chips. Clean glass plates with ethanol and lint-free cloths before use. The height of the stacking gel should be at least 2x the height of the sample in the well. This ensures band sharpness, even for diluted protein samples. Store gels flat in the fridge at 4°C.

    Get Price
  • Denaturing Polyacrylamide/Urea Gel Electrophoresis

    2018-8-22 · gel plates to the top of the electrophoresis tank and ll the upper reservoir with 1X TBE so that the wells are covered. 8. Pre-run and warm the gel for at least 30 min at 5 V/cm (constant voltage). Note Heat the gel (buffer) during the whole run at 60-70°C. 9. Wash the wells with 1X TBE buffer to remove UREA and gel pieces. 10. Load the samples. 11.

    Get Price
  • Polyacrylamide Gel Electrophoresis (PAGE ...

    2018-10-20 · Principle of Polyacrylamide Gel Electrophoresis (PAGE) SDS-PAGE (Polyacrylamide Gel Electrophoresis), is an analytical method used to separate components of a protein mixture based on their size. The technique is based upon the principle that a charged molecule will migrate in an electric field towards an electrode with opposite sign.

    Get Price
  • polyacrylamide gel recipe pam supplier,

    Alibaba.com offers 1,560 polyacrylamide gel recipe pam supplier products. A wide variety of polyacrylamide gel recipe pam supplier options are available to you,

    Get Price
  • polyacrylamide gel recipe, polyacrylamide gel recipe ...

    Alibaba.com offers 1,575 polyacrylamide gel recipe products. A wide variety of polyacrylamide gel recipe options are available to you,

    Get Price
  • polyacrylamide gel recipe polyacrylamide gel recipe ...

    2021-6-2 · Neutral Polyacrylamide Gel - Semantic Scholar. Work quickly to complete the gel before the acrylamide polymerizes. Add 35 µl of TEMED for each 100 ml of acrylamide:bis solution, and mix the solution by gentle swirling.

    Get Price
  • ExpressPlus TM PAGE Gels, 10×10 - GenScript

    2016-9-28 · High reproducibility – Guaranteed consistent performance of each gel Cost effective – Significant reduction in the cost of each ... Gel material Polyacrylamide Polyacrylamide Gel dimensions 7.4 cm×8.4 cm(H×W) 8.6 cm×8.0 cm(H×W) Gel thickness 1.0 mm 1.0 mm Resolving gel height 6 cm 6.5 cm Cassette dimensions 8.4 cm×10.0 cm(H×W) 10.2 cm ...

    Get Price
  • 3.15. Handcasting Polyacrylamide Gels | Markelz

    Measure 5 ml of resolving gel into a centrifuge tube. Add 2.5 μl of TEMED and 25 μl of 10% APS to the tube. Cap the tube and gently mix the solution by inverting the test tube. Fill the glass cassette to the mark made in step 5 with resolving gel using a pippette. Add about 100 μl …

    Get Price
  • SDS-PAGE Gel Recipes | Proteintech Group

    In order to target proteins with MWs between 20 and 200 kDa, you will need to create a conventional SDS-PAGE gel using the recipes shown below. The percentage of gel you require corresponds with the MW of your target protein. Dissolve compounds thoroughly. Adjust pH slowly to pH 8.8 with concentrated HCl, then add ddH2O to 1000ml.

    Get Price
  • DNA Polyacrylamide Gel Electrophoresis

    2013-11-4 · DNA Polyacrylamide Gel Electrophoresis How to pour and run a neutral polyacrylamide gel. Buffers and Solutions Acrylamide:bisacrylamide (29:1) (30% w/v) Ammonium persulfate (10% w/v) Ammonium persulfate is used as a catalyst for the copolymerization of acrylamide and bisacrylamide gels. The polymerization reaction is driven by free

    Get Price
  • Running agarose and polyacrylamide gels

    2010-4-20 · 20 Polyacrylamide Gel Recipe For Dna. Smeared dna bands in polyacrylamide gels but not agarose gel possible reasons 1 recipe of polyacrylamide gels table introduction to polyacrylamide gels lsr bio rad acrylamide gel electropsis thermo fisher scientific us.

    Get Price
  • 20 Polyacrylamide Gel Recipe For Dna | Deporecipe.co

    2018-8-21 · 9. Run the gel at 5 V/cm, taking care to avoid excessive heating. Run the gel for the time indicated in the certificate of analysis of the ladder. 10. Stain the gel in a 0.5 µg/ml ethidium bromide aqueous solution for about 30 min. Examine the gel under the UV light. This protocol is for the Non-denaturing PAGE

    Get Price
  • polyacrylamide gel recipe, polyacrylamide gel recipe ...

    Alibaba.com offers 1,575 polyacrylamide gel recipe products. A wide variety of polyacrylamide gel recipe options are available to you,

    Get Price
  • polyacrylamide gel recipe polyacrylamide gel recipe ...

    2021-6-2 · Neutral Polyacrylamide Gel - Semantic Scholar. Work quickly to complete the gel before the acrylamide polymerizes. Add 35 µl of TEMED for each 100 ml of acrylamide:bis solution, and mix the solution by gentle swirling.

    Get Price
  • Polyacrylamide gel analysis of oligonucleotides

    5. Load the samples onto the gel. Also load a separate well with 1x formamide loading buffer containing xylene cyanol FF and bromophenol blue. On a 15% polyacrylamide gel, these marker dyes co-migrate with oligonucleotides with lengths of 30 and 9–10 bases respectively. 6. Run the gel for 1.5 …

    Get Price
  • SDS and native polyacrylamide gel electrophoresis of

    2014-7-15 · SDS and native polyacrylamide gel electrophoresis of proteins Supplies and Reagents Acrylamide solutions (see Table 1 and Table 2 for recipes) ... Components Gel Volume => 5 ml 10 ml 15 ml 20 ml 25 ml 30 ml 40 ml 50 ml 6% gel H 2O 2.6 5.3 7.9 10.6 13.2 15.9 21.2 26.5 30% acrylamide mix 1.0 2.0 3.0 4.0 5.0 6.0 8.0 10.0 ...

    Get Price
  • 12 Polyacrylamide Gel Recipe | Besto Blog

    2018-7-26 · Precast Gel Sds Page 4 20 8x10cm 12well Ab119209 Abcam Introduction to polyacrylamide gels lsr bio rad protocol for making sds recipe for polyacrylamide gel 1 solutions for preparing resolving gels tris glycine sds page. Whats people lookup in this blog: 12 Polyacrylamide Gel Recipe; 12 Sds Gel Recipe; 12 Sds Page Gel Recipe

    Get Price
  • Denaturing Polyacrylamide Gel Recipe Besto Blog ...

    We've gathered our favorite ideas for Denaturing Polyacrylamide Gel Recipe Besto Blog, Explore our list of popular images of Denaturing Polyacrylamide Gel Recipe Besto Blog and Download Every beautiful wallpaper is high resolution and free to use. Download for free from a curated selection of Denaturing Polyacrylamide Gel Recipe Besto Blog for your mobile and desktop screens.

    Get Price
  • 12 polyacrylamide gel | Industrial water treatment ...

    12 Polyacrylamide Gel Recipe | Bryont Rugs and Livings. Sds page gel recipes sample page recipes if you this sheet can change the acrylamide percentages and batch sizes will auto update sds page gel preparation recipe table How Do You Choose Gel Percentage For Electropsis Western -> Source : …

    Get Price
  • 20 Polyacrylamide Gel Recipe For Dna | Deporecipe.co

    2010-4-20 · 20 Polyacrylamide Gel Recipe For Dna. Smeared dna bands in polyacrylamide gels but not agarose gel possible reasons 1 recipe of polyacrylamide gels table introduction to polyacrylamide gels lsr bio rad acrylamide gel electropsis thermo fisher scientific us.

    Get Price
  • 6 Polyacrylamide Gel Recipe | Deporecipe.co

    6 Polyacrylamide Gel Recipe. 6 recipe for running portion of polyacrylamide gel volumes are scientific diagram recipe for polyacrylamide gel sds page gel preparation recipe table recipe for polyacrylamide gel. 6 Recipe For Running Portion Of Polyacrylamide Gel Volumes Are Scientific Diagram

    Get Price
  • benefits of 10 polyacrylamide gel recipe besto blog ...

    PolyacrylamideGelRecipe | Besto Blog. 2 separation polyacrylamide gel recipes for two gels very low molecular weight proteins electropsis protocol recipe for sds page gels 6 recipe for running portion of polyacrylamide gel volumes are Whats people lookup in this blog:

    Get Price
  • SDS polyacrylamide gel - Pennsylvania State University

    2003-1-25 · SDS polyacrylamide gel. Assemble the gel plates with spacers that match the thickness of the comb you plan to use. Clamp the glass sandwich with black clamps. Locate a comb of matching thickness. Seal the edges of the glass sandwich with molten agarose; this is easier than sealing with tubing and cleaner than grease. Prepare the running gel.

    Get Price
  • 10% Acrylamide Gels for SDS-PAGE

    2008-5-23 · 5 ml 10% SDS Pouring resolving gel: 1. Make 6 ml of resolving gel (makes 1 gel, with a little bit leftover) 3.96 ml of resolving gel master mix 1.98 ml of 30% acrylamide 60 μl of 10% APS 2.4 μl of TEMED 2. Immediately load gel mixture into the casing with a pipette – fill to the line on the casing. Add EtOH on top of gel. Save any leftover ...

    Get Price
  • SDS PAGE-Preparation

    2014-7-1 · The SDS PAGE gel in a single electrophoresis run can be divided into stacking gel and separating gel. Stacking gel (acrylamide 5%) is poured on top of the separating gel (after solidification) and a gel comb is inserted in the stacking gel. The acrylamide percentage in SDS PAGE gel depends on the size of the target protein in the sample.

    Get Price
  • The principle and method of polyacrylamide gel ...

    Polymerized acrylamide (polyacrylamide) forms a mesh-like matrix suitable for the separation of proteins of typical size. The strength of the gel allows easy handling. Polyacrylamide gel electrophoresis of SDS-treated proteins allows researchers to separate proteins based on their length in an easy, inexpensive, and relatively accurate manner.

    Get Price
  • Polyacrylamide Gel Electrophoresis (PAGE)

    2006-12-13 · POLYACRYLAMIDE GEL ELECTROPHORESIS (PAGE) Last Update: December 2006. PREPARE SOLUTIONS. 1. 30% Acrylamide 0.8% Bis (300 mL): Mix 90 g of Acrylamide, 2.4 g of N'N'-bis-methylene-acrylamide, and 300 mL of dH 2 O (filter through a 0.45 m filter) (Store at 4 o C in a dark bottle) 2. 10% SDS (100 mL): Weigh 10 g of SDS and add dH 2 O to 100 mL.

    Get Price
  • SDS-PAGE

    2003-8-18 · in the resolving gel. 5] Allow resolving gel to stand 30 min at room temperature. 6] Drain the DDI H 2O from top of the resolving gel. Rinse with DDI H 2O, drain, and wick any remaining DDI H 2O away with a Kimwipe. 7] Mix components for stacking gel. 8] Pour stacking gel solution into gel plates (on top of running gel), so that gel plates are ...

    Get Price
  • Protocol Online: Denaturing Urea-Polyacrylamide Gel ...

    2019-12-20 · Denaturing Urea-Polyacrylamide Gel Electrophoresis (PAGE) Based Microsatellite Analysis: Author: Sanjeev Sharma 1, BR Yadav 1, Affiliation: 1 Livestock Genome Analysis Lab, 3 Animal Biochemistry Division, National Dairy Research Institute, Karnal-132001, India 2 Meerut Institute of Engeenering and Technology, Meerut, U.P., India: Date Added: Mon Feb 02 2009 Date Modified: Mon …

    Get Price
  • Food Cost Formula: How to Calculate Food Cost

    2021-5-13 · PoE 5 Links & Sockets Recipe. 1. Socket and Link Currency. Jeweller's Orb re-rolls the number of sockets on an item. The Minimum Level of five sockets is 35. The chance of getting five sockets is about 1.63%. The item's quality increases the chances of obtaining more sockets. Orb of Fusing reforges the links between sockets on an item.

    Get Price
  • 12 Sds Page Gel Recipe 30 Acrylamide | Amtrecipe.co

    12 Sds Page Gel Recipe 30 Acrylamide. masuzi September 17, 2019. How do you choose gel percentage for electropsis western blot sds page gels sds western webpage powered by ramaz geguchadze introduction to polyacrylamide gels lsr bio rad. ... Recipe For Polyacrylamide Gel

    Get Price
  • High Speed Protein Separation by SDS-PAGE

    2021-6-24 · The 3.5, 10, and 15 kDa proteins co-migrated and were not distinguishable. However, the same gel run with FASTRun buffer showed complete separation of these lower MW proteins (Fig. 3b). Moreover, the separation range in this 10% TG gel using FASTRun buffer was equal to typical runs using 4–20% gradient gels. Table 3.

    Get Price
  • Polyacrylamide hydrogel phantoms for performance ...

    PAA phantoms were fabricated from a 400 mL batch of solution using a final recipe of 12 % w/v acrylamide, 0.08 % w/v APS, 0.2 % v/v TEMED, 0.7 mg/mL TiO 2, 0.00225 % v/v India ink, and 6 mg/mL glass beads. Phantoms took approx. 5 min to solidify and were allowed to cure for 1 h, with a maximum surface temperature of 46 °C as measured by an ...

    Get Price
  • Reusable heat-sensitive phantom for precise estimation

    2018-6-9 · gel (semisolid) forms; but the gel phantoms have found more ... The low cost of preparation due to the low ... the proposed recipe is only valid for ablative temperature ranges (45–55 C), and it is not applicable for mild hyper-thermia application that applies a lower level of temperature (40–45 C) [36]. Therefore, in order to construct an ...

    Get Price
  • Amazon.com: polyacrylamide crystals

    Prestige Import Group Water Gathering Polymer Crystals - Superabsorbent Cross-Linked Polyacrylamide Helps Store Water & Nutrients - 1 lb. 4.2 out of 5 stars. 4. 15.49. 15. . 49. Get it as soon as Mon, Jun 28. FREE Shipping on orders over 25 shipped by Amazon.

    Get Price
  • Barrick Lab :: Laboratory Protocols

    Protocols for Qubit, Nanodrop, and Agarose Gel DNA concentrations, and example data highlighting caveats for each. DNA Sequencing; 16S rRNA Sequencing ... Master mix recipe, procedure, and Gibson walkthrough included. ... Polyacrylamide Gel Electrophoresis Denaturing and nondenaturing (native) polyacrylamide gel electrophoresis. ...

    Get Price
  • PROCESS FOR PRODUCING AQUEOUS

    A process for producing an aqueous polyacrylamide gel comprising polymerizing an aqueous solution comprising at least acrylamide thereby obtaining an aqueous polyacrylamide gel, wherein the process comprises at least the following steps: [1] Preparing an aqueous monomer solution comprising at least water, 5% to 45% by weight—relating to the ...

    Get Price
  • US6726821B1 - Polyacrylamide precast gels for ...

    A precast acrylamide electrophoresis gel is disclosed, which includes a gel buffer solution containing either Tris and glycine or Tris, glycine and any one or more than two of ampholytes, and a monobasic acid, titrated to a specified pH range. With the gels constituted as stated earlier, proteins can be successfully subjected to electrophoresis using the commonly available Laemmli's buffer ...

    Get Price
  • Dissolvable Polyacrylamide Beads for

    2020-2-20 · The inDrop polyacrylamide bead can be closely packed in a microfluidic device channel to achieve more than 95% loading of single bead per drop. 2 However, in the inDrop system, UV light is necessary to release primers from the bead, which somewhat complicates bead fabrication and makes it less cost effective. 9 Another shortcoming is that UV ...

    Get Price
  • CHAPS Detergent: Protocols and Frequently Asked

    The use of a zwitterionic detergent in two-dimensional gel electrophoresis of trout liver microsomes, 1983, Anal. Biochem., v. 135, 453-455; Schupbach, J., et al., A universal method for two-dimensional polyacrylamide gel electrophoresis of membrane proteins using isoelectric focusing on immobilized pH gradients in the first dimension, 1991, Anal.

    Get Price
  • DNA Polyacrylamide Gel Electrophoresis

    2013-11-4 · DNA Polyacrylamide Gel Electrophoresis How to pour and run a neutral polyacrylamide gel. Buffers and Solutions Acrylamide:bisacrylamide (29:1) (30% w/v) Ammonium persulfate (10% w/v) Ammonium persulfate is used as a catalyst for the copolymerization of acrylamide and bisacrylamide gels. The polymerization reaction is driven by free

    Get Price
  • Horse joint treatment Gel - NOLTREX Vet

    Noltrex®Vet (4.0% Polyacrylamide) is a highly viscous, non-soluble, synthetic hydrogel for intra-articular injection. The hydrostatic pressure inside the joint presses the gel up against the inner linings of the joint where it forms a fine, lubricating film.

    Get Price
  • SDS-PAGE PROTOCOL Adapted from Current Protocols,

    2006-2-1 · 0.5 M Tris, pH 6.8 5 ml 50% Glycerol 8 ml 10% SDS 8 ml 2-βmercaptoethanol 2 ml (add immediately before use) bromophenol blue 10% (v/v) acetic acid Protocol 1. Prepare polyacrylamide gel according to standard protocol. 2. Load samples and run gel @ 25 mA (2 gels run @ 50 mA) in 1x SDS Running Buffer. 3.

    Get Price
  • Preparing SDS gels - Rice University

    2017-6-7 · !!Prepare 0.01µg/µl stock by mixing 5 ul of 1 µg/µl ladder + 360 µl of 10mM Tris-Cl pH 8.5 (you can use leftover EB buffer from the Qiagen kits!). •!Run the gel at either 100 or 125 constant volts for 45-90 min (keep an eye on the dye front, for standard plasmid gels with digests let the dye travel 2/3 or more to the end of the gel before

    Get Price
  • Agarose Gel Protocol - University of San Diego

    2013-9-24 · Laemmli’s system for polyacrylamide gel protein electrophoresis in the presence of the detergent SDS (SDS/PAGE) is one of the most cited methodological papers in life sciences ().The facility with which SDS/PAGE resolves minute amounts of proteins revolutionized the analysis of tissue and cell extracts, resulting in “overnight” adoption of the technique in biochemistry, cell biology ...

    Get Price
  • Using Polyacrylamide (PAM) to Reduce Erosion on ...

    2020-4-21 · The volumes provided in each column are for approximately 25 mL of separating gel, enough for four 1.0 mm thick mini gels. Scale volumes proportionally based on the number of gels to be cast. Stacking gel The following recipe is for a 4% stacking gel (12.5 mL). Solution 6% gel 8% gel 10% gel 12% gel 14% gel 16% gel 18% gel 20% gel

    Get Price
  • Acrylamide concentration determines the direction

    2021-7-8 · Calculate the volume of gel-ready sample to load in each well. Now that you know the concentration of the sample you'll be loading on the gel, you can calculate the volume to pipet into the well. The recommended mass of protein may vary for different experiments, but for your first gel with fish muscle proteins, try to get 4 μg protein in each ...

    Get Price
  • Protocol: Protein electrophoresis and western blot recipes

    Choose from over 900 colors of premium polyester gel coat, color matched every time you order. Both RAL and Instint® lines feature UV and blister resistance, with excellent adhesive properties. We custom mix your color and still ship same day! Order quart, gallon, and 5-gallon sizes. Color books are available for purchase or rent.

    Get Price
  • SDS-PAGE - Assay-Protocol

    2019-7-31 · Download SDS-PAGE protocol as a PDF . SDS-PAGE, with full name of sodium dodecyl sulfate polyacrylamide gel electrophores, is the most widely used technique to separate proteins from complicated samples of mixture, plays key roles in molecular biology and wide range of subfield of biological research. Being present a electricity, proteins migerate towards the negative anode inside …

    Get Price
  • Tris-Glycine vs Bis-Tris Gel Chemistry | Abcam

    Migration Chart for RunBlue™ Bis-Tris Precast Gel with MES MOPS Buffer. References: Graham, DRM., Garnham, CP., Fu, Q., et al. Improvements in two-dimensional gel electrophoresis by utilizing a low cost “in-house” neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis system. Proteomics. 5(9), 2309–2314 (2005).

    Get Price
  • Food Cost Calculator – Recipe Costing Software

    The total recipe costs 24.16 and you will notice our software captures ingredients, labor, and materials in this recipe. 3 cups of this béchamel sauce cost 4.54 this is huge to know that your labor and materials were captured in the sub recipe. These are real costs that need to be recorded.

    Get Price
  • US6726821B1 - Polyacrylamide precast gels for ...

    A precast acrylamide electrophoresis gel is disclosed, which includes a gel buffer solution containing either Tris and glycine or Tris, glycine and any one or more than two of ampholytes, and a monobasic acid, titrated to a specified pH range. With the gels constituted as stated earlier, proteins can be successfully subjected to electrophoresis using the commonly available Laemmli's buffer ...

    Get Price
  • Food Cost Calculator to Ensure Your Restaurant ...

    Calculating Recipe and Sub Recipe Cost. Your recipe cost is the cost of the ingredients for each serving of a dish. Sometimes it is easier to calculate the recipe cost for multiple servings, then divide that cost by the number of servings. Sourcery’s example uses the ingredient cost of …

    Get Price
  • Recipe Cost Calculator for Excel - Spreadsheet123

    2020-10-25 · Using AccuChef recipe software you can resize your recipes for any number of servings. Use its built in coupon database to organize your coupons. You can use AccuChef recipe software to perform a nutritional analysis on your recipes, cost your recipes, print out index cards, email recipes to your friends and design your own customized output.

    Get Price
  • Recipe Software | AccuChef v6.7

    2021-7-7 · 5.08 recipe 0.64 per cup While red cabbage is still on sale for 0.50/lb., I’m just going to keep going with it! This super simple Sesame Slaw is fast, fresh, and a …

    Get Price