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best sell cationic polyacrylamide gel protocol

  • Silver staining DNA in polyacrylamide gels | Nature

    2007-10-25 · Prepare sufficient formaldehyde solution (as described in REAGENT SETUP) to cover the gel in the staining tray to a depth of ∼ 5 mm. 9. Prepare sufficient silver solution (as described in ...

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  • how can i dissolve a polyacrylamide gel researchgate

    basic protocol: purification of oligonucleotides using denaturing polyacrylamide gel electrophoresis For example,while a 20 % gel can be electrophoresed at 800 V with few problems, an8 % gel under the same conditions would likely generate too much heat forthe apparatus to dissipate.

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  • Silver staining of proteins in polyacrylamide gels ...

    2006-11-22 · With gloved hands, rinsed with deionized water, pull one gel out of the silver solution. Dip it for 10 s in the water bath, then pull it out of the water and transfer it to the basic developer ...

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  • Polyacrylamide Gel Crystals - Home Science Tools

    Water gel crystals, also known as water jelly crystals and superabsorbent polymer, are able to absorb up to 400 times their weight in water. Place 1 teaspoon (4g) of these small 2-4mm crystals in a quart of water, let it sit overnight, pour off the excess water and you will have over 2 cups of beautiful, large 1-2' jelly-like clear crystals.

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  • Analysis of DNA by Agarose Gel Electrophoresis

    Abstract. Electrophoresis through agarose or polyacrylamide gels is used to separate, analyze, identify, and purify DNA fragments. The technique is simple, rapid to perform, and capable of resolving fragments of DNA that cannot be separated adequately by other procedures, such as density gradient centrifugation.

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  • PAGE Gel | Bio-Rad

    2018-5-18 · Cationic polyacrylamide copolymers (PAMs) are a group of water-soluble polymers with a wide range of applications in industry, food processing, agriculture and waste management. One of the major applications for PAM is sludge dewatering in municipal waste water treatment plants (MWWTPs).

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    2014-9-8 · Acid-urea polyacrylamide gel electrophoresis (AU-PAGE) followed by Western immunoblotting is an important tool for the identification and quantification of cationic AMPs. The protocol for these procedures outlined here describes, in detail, the necessary steps; including pouring the AU-gels, preparing the test samples, performing the electrophoretic separation and protein transfer to the …

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  • Detection of Antimicrobial (Poly)Peptides with Acid

    2011-4-25 · Cite this protocol as: Barth J., Volknandt W. (2011) Two-Dimensional BAC-SDS Polyacrylamide Gel Electrophoresis for the Fractionation and Identification of Synaptic Vesicle Proteins and the Presynaptic Active Zone. In: Li K. (eds) Neuroproteomics. Neuromethods, vol 57. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-111-6_9

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  • how can i dissolve a polyacrylamide gel researchgate

    basic protocol: purification of oligonucleotides using denaturing polyacrylamide gel electrophoresis For example,while a 20 % gel can be electrophoresed at 800 V with few problems, an8 % gel under the same conditions would likely generate too much heat forthe apparatus to dissipate.

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  • Polyacrylamide Gel Electrophoresis - an overview ...

    Polyacrylamide Gel Electrophoresis. SDS polyacrylamide gel electrophoresis shows that the 30S protein complexes of mammalian skeletal and cardiac muscles are composed of a single major high molecular weight RyR polypeptide and isoform-specific low molecular weight immunophilin (FK506 binding protein) which migrate with apparent Mr > 340000 (see Fig. 45.12B) and Mr 12000 (not visible on the ...

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  • (PDF) A Guide to Polyacrylamide Gel Electrophoresis

    For protein separation, virtually In gel electrophoresis, proteins do not all enter the all methods use polyacrylamide as an anticonvective, gel matrix at the same time. Samples are loaded sieving matrix covering a protein size range of into wells, and the proteins that are closer to the gel 5–250 kD.

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  • Cationic polyacrylamide copolymers (PAMs):

    2018-5-18 · Cationic polyacrylamide copolymers (PAMs) are used for sludge dewatering in municipal waste water treatment and might enter the environment by spreading of the sludge on agricultural land. Concern has been expressed since little is known about the degradation of PAMs in soils. To obtain detailed information on the polymer’s fate in the soil compartment, the degradation of 14C …

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  • Xitao Polymer Co., Ltd. - Polyacrylamide & Super

    Cationic Polyacrylamide Polymer Powder(C5015) for sludge dewatering in waste water treatment FOB Price: USD 3.85 Kilogram Min. Order: 1000 Kilograms; Belt press cationic polyacrylamide polymer floccluant FOB Price: USD 3.75 Kilogram Min. Order: 1000 Kilograms; Medium cationic charge polyacrylamide (C1305) for air flotation application

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  • Coomassie Blue (R-250, G-250)

    2020-11-2 · Standard Gel staining Protocol 1- Gel may be prefixed in 50% MeOH, 10% HoAC, 40% H 2 O for 30 minutes to overnight. 2- Stain gel in the above solution, with 0.25-0.3% Coomassie Blue R-250, for 2 - 4 hours, until the gel is a uniform blue color. Staining is complete when the gel …

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  • Advanced Methods in Molecular Biology and

    A step-by-step protocol will help the students and researchers to follow the procedure efficiently and effectively. Select Chapter 3 - SDS-polyacrylamide gel electrophoresis. Book chapter Full text access. Chapter 3 - SDS-polyacrylamide gel electrophoresis. ... Cationic liposomes that form nanoparticle complexes with DNA and RNA are widely used ...

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  • High efficient cationic - sizwematrix.co.za

    high efficient cationic&anionic PAM,CPAM,polyacrylamide powder&crystals&gel&granules copolymer for water treatment . Polyacrylamide is short for PAM, which is a glassy solid. It is easy to absorb water, the speed of the adsorption should be different according to the features of the ramification plasma.

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  • Polyacrylamide Gel Crystals - Home Science Tools

    Water gel crystals, also known as water jelly crystals and superabsorbent polymer, are able to absorb up to 400 times their weight in water. Place 1 teaspoon (4g) of these small 2-4mm crystals in a quart of water, let it sit overnight, pour off the excess water and you will have over 2 cups of beautiful, large 1-2' jelly-like clear crystals.

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    2021-6-20 · China Powder Silica Gel, China Powder Silica Gel Manufacturers and Sup.... Order) Contact Supplier Best Price 75% benzoyl peroxide for acne cosmetic benzoyl peroxide Qingdao Sigma Chemical Co., Ltd. US 60.00 Kilogram 1 Kilogram (Min. Order) Contact Supplier Nonionic Polyacrylamide …

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  • Xitao Polymer Co., Ltd. - Polyacrylamide & Super

    Cationic Polyacrylamide Polymer Powder(C5015) for sludge dewatering in waste water treatment FOB Price: USD 3.85 Kilogram Min. Order: 1000 Kilograms; Belt press cationic polyacrylamide polymer floccluant FOB Price: USD 3.75 Kilogram Min. Order: 1000 Kilograms; Medium cationic charge polyacrylamide (C1305) for air flotation application

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  • Coomassie Blue (R-250, G-250)

    2020-11-2 · Standard Gel staining Protocol 1- Gel may be prefixed in 50% MeOH, 10% HoAC, 40% H 2 O for 30 minutes to overnight. 2- Stain gel in the above solution, with 0.25-0.3% Coomassie Blue R-250, for 2 - 4 hours, until the gel is a uniform blue color. Staining is complete when the gel …

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    The method of Polyacrylamide production technology. Emulsion polymerization is another important synthesis method polyacrylamide, which is a conventional operation in the dispersed aqueous acrylamide solution in an organic solvent such as gasoline, with vigorous stirring, the solution was uniformly dispersed emulsion system is formed, followed by the addition of acrylamide initiator reaction ...

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  • Gels | Special Issue : Cryogelation and Cryogels

    2019-8-31 · Dear Colleagues, Cryogelation, a polymerization process intended to form a crosslinked and macroporous gel network at sub-zero temperatures, has initially been used only for a handful of polymers since the 1970s. Gels produced with this process are known as freeze-thawed cryogels, or more commonly cryogels.

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  • How do we deal with an accidental flocculant spill ...

    2021-4-12 · Flocculants are polymers that are mainly used in the treatment of wastewater containing suspended particles. During a spill, the ground surface soiled by flocculants becomes extremely slippery. If cleaning is not carried out perfectly, mere contact with water can cause a serious fall hazard. In this article, discover the nature of flocculants, where they are used and our spill management advice.

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  • CHAPS Detergent: Protocols and Frequently Asked

    A nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents. Commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing.

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    cationic polyacrylamide coagulants flocculants, cationic polyacrylamide coagulants flocculants Suppliers and Manufacturers at Okchem. cationic polyacrylamide Specification For municipal and industrial waste water treatment. storage Keep away from straight sunshine, wet air and high temperature, keep the air dry and ventilated. laboratory factory Application For more safety

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    Wholesale Cationic Polyacrylamide Used in Textile Printing. Wholesale Cationic Polyacrylamide Used in Textile Printing Water Treatment Polyacrylamide is a linear polymer, it is soluble in water, almost insoluble in benzene, ethylbenzene, esters, acetone and other general organic solvents, the water solution is almost transparent viscous liquid, is a non-dangerous goods , Non-toxic, non ...

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  • Sell - Cationic Polyacrylamide

    Cationic polyacrylamide, copolymerized by cationic monomer and acrylamide, is a kind of water-soluble polyelectrolyte, which is nontoxic, tasteless, soluble in water easily and insoluble in organic solvents, such as ethanol and acetone. The molecular chain is flexible, relative molecular wight is steady, ionic distribution is even.

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    Buy cationic polymer polyacrylamide for wastewater treatment direct from Cationic polyacrylamide of China Factory that provide Latest Cationic polyacrylamide - pam-company. ... We can assure that we provide best products, reasonable price and perfect service. ... and can be cross-linked into an insoluble gel with the high chemical price metal ...

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    China Polyacrylamide Gel Electrophoresis Pdf. Polyacrylamide Gel Electrophoresis Pdf Manufacturers, Factory, Suppliers From China, Our tenet is clear all the time: to deliver high quality product at competitive price to customers around the world. We welcome potential buyers to …

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    Sell cationic polyacrylamide with drilling based mud chemicals additives(id:24242714), China manufacturer, supplier, exporter, Puyang Rongtai Chemical Co., Ltd. Source for polyacrylamide price, WATER TREATMENT CHEMICALS, flocculant powder here.

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    Cationic Polyacrylamide Price, Wholesale & Suppliers. There are 1,417 cationic polyacrylamide price suppliers, mainly located in Asia. The top supplying countries or regions are China, Thailand, and Ukraine, which supply 99%, 1%, and 1% of cationic polyacrylamide price respectively.

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    Polyacrylamide (PAM) Anionic Cationic is technically named Polyacrylamide, which is a water soluble macromolecular polymer. white fine-sand shaped powder or colorless transparent colloid. 1.Used as flocculent to separate solid from liquid in the fields of mineral separation, metallurgy, chemicals and …

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    Best Selling Nonionic Polyacrylamide Used In. Cationic polyacrylamide selling leads - ec21 sell cationic polyacrylamide with drilling based mud chemicals additives.The cationic polyacrylamide cpam is a linear polymer compound, and since it has various active groups, it can affinity with many substances and adsorb to form hydrogen bonds.

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  • Interaction of Cationic Nickel and Manganese

    2019-8-17 · Experimental protocol for the synthesis of TMPyP porphyrins, 13 C NMR data for meso-5,10,15,20-tetrakis(4-(N-methyl-pyridinium-2-yl)phenyl)porphyrin tetrachloride. Polyacrylamide gel electrophoresis analysis of ds19−3AT cleavage corresponding to Figure …

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    Polyethylenimine, Linear, MW 250,000 (PEI 250000)Catalog Number 24314. Linear polyethylenimine (PEI) is a high-charge cationic polymer that readily binds highly anionic substrates. Industrially, linear PEI can improve the appearance of negatively charged dyes by modulating their properties and improving their adherence to surfaces. View Product.

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  • Polyethylenimine (PEI) - Polymers - Products

    2018-6-4 · DNA & RNA Precipitation Solutions For research use only. Not for use in diagnostic procedures for clinical purposes. PS40-5132_PptionSoln_Ver3.1.doc l www.genelink.com l Page 2 of 14

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  • DNA & RNA Precipitation Solutions - Gene Link

    2020-7-20 · Expression and purification analysis. a Shows a sodium dodecyl sulfate–polyacrylamide gel (SDS − PAGE) analysis to confirm the expression of anti-G17-Gly. The identical amount of samples including UN, uninduced total cell lysate; IN, induced total cell lysate; UNP, uninduced periplasm fraction; and INP, induced periplasmic fraction were separated on 12% SDS-PAGE and then stained with ...

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  • Optimization of Tris/EDTA/Sucrose (TES) periplasmic ...

    Figure Legend Snippet: Comparison between Pt and PEDOT:PSS electrodes – gel images. Images of the Rainbow Marker separated in an 11-mm-wide PHAST PAGE gel showing the equivalence of the separation of GE Healthcare’s Full Range Rainbow Recombinant Protein Molecular Weight Marker driven with PEDOT:PSS electrodes (left) and with Pt electrodes (right).

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  • DsiRNAs and TriFECTa RNAi Kits - Integrated DNA

    2011-7-15 · Protein extraction is a frequent procedure in biological research. For preparation of plant cell extracts, plant materials usually have to be ground and homogenized to physically break the robust cell wall, but this step is laborious and time-consuming when a large number of samples are handled at once. We developed a chemical method for lysing Arabidopsis cells without grinding.

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  • Full Range Rainbow Recombinant Protein Molecular

    2021-5-14 · That is a big reason for the wide use of SDS polyacrylamide gel electrophoresis (SDS-PAGE) for separating and studying proteins. Usually, for complete cell lysis in the presence of SDS, a sample must be sonicated or sheared (e.g., passed through a 19G …

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  • Full Range Rainbow Recombinant Protein Molecular

    Figure Legend Snippet: Comparison between Pt and PEDOT:PSS electrodes – gel images. Images of the Rainbow Marker separated in an 11-mm-wide PHAST PAGE gel showing the equivalence of the separation of GE Healthcare’s Full Range Rainbow Recombinant Protein Molecular Weight Marker driven with PEDOT:PSS electrodes (left) and with Pt electrodes (right).

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  • Polyethylenimine (PEI) - Polymers - Products

    Polyethylenimine, Linear, MW 250,000 (PEI 250000)Catalog Number 24314. Linear polyethylenimine (PEI) is a high-charge cationic polymer that readily binds highly anionic substrates. Industrially, linear PEI can improve the appearance of negatively charged dyes by modulating their properties and improving their adherence to surfaces. View Product.

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    2019-3-27 · maintenance protocol Key Words Stock culture maintenance, microbiology, quality control ... This document highlights industry guidelines and best practices to ensure compliance to various standards, at the ... ready-to-use bacteriological loops containing gel-stabilised micro-organisms. Each loop is individually packaged in a foil

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    2020-4-30 · Best Practices - Technical Note. This technical note describes best practices for using an Agilent LC system. Contents. Housekeeping . 2. How to deal with solvents. 2 How to prepare samples . 3. Daily Weekly tasks. 4 Power up Shut-down the system . 5. Power-up the system. 5 …

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  • A tumor-to-lymph procedure navigated versatile gel

    2018-4-17 · Natural medicines were the only option for the prevention and treatment of human diseases for thousands of years. Natural products are important sources for drug development. The amounts of bioactive natural products in natural medicines are always fairly low. Today, it is very crucial to develop effective and selective methods for the extraction and isolation of those bioactive natural products.

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    2007-8-21 · We developed a simple method that is able to provide label-free sequence-specific DNA detection with single-nucleotide polymorphism (SNP) detection selectivity. This method makes use of both DNA probe immobilized silica nanoparticles and optically amplifying light harvesting molecules. The recognition is accomplished by sequence-specific hybridization between the DNA probes on the silica ...

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  • Polyacrylamide Gel Crystals - Home Science Tools

    Water gel crystals, also known as water jelly crystals and superabsorbent polymer, are able to absorb up to 400 times their weight in water. Place 1 teaspoon (4g) of these small 2-4mm crystals in a quart of water, let it sit overnight, pour off the excess water and you will have over 2 cups of beautiful, large 1-2' jelly-like clear crystals.

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  • nonionic polyacrylamide method of operation new

    The polyacrylamide has a wide model range, the most common models are the anionic polyacrylamide, the cationic polyacrylamide and the nonionic polyacrylamide. The effective PH value is different to different types of polyacrylamide, so in the selection of the polyacrylamide, the influence of the PH value to the polyacrylamide must be considered.

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  • Precast Gradient Gel | Bio-Rad | Bioz

    Bio-Rad precast linear gradient tris hcl polyacrylamide gel Precast Linear Gradient Tris Hcl Polyacrylamide Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

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  • Interaction of Cationic Nickel and Manganese

    2019-8-17 · Experimental protocol for the synthesis of TMPyP porphyrins, 13 C NMR data for meso-5,10,15,20-tetrakis(4-(N-methyl-pyridinium-2-yl)phenyl)porphyrin tetrachloride. Polyacrylamide gel electrophoresis analysis of ds19−3AT cleavage corresponding to Figure …

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  • CHAPS Detergent: Protocols and Frequently Asked

    CHAPS (formal name: 3-cholamidopropyl dimethylammonio 1-propanesulfonate) is a non-denaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. This detergent combines the useful properties of both sulfobetaine-type and the bile salt detergents. CHAPS is commonly used for protein solubilization ...

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  • How do we deal with an accidental flocculant spill ...

    2 天前 · Gel-filtration chromatography (Basic Protocol 2) is not generally considered a high-resolution separation technique. However, as the recombinant-derived protein content of well-prepared washed pellets will be >50% of the total (see Fig. 6.3.1 , lanes h and i), only a 2-fold purification is required to obtain pure protein.

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  • Preparation and Extraction of Insoluble (Inclusion

    2007-8-21 · We developed a simple method that is able to provide label-free sequence-specific DNA detection with single-nucleotide polymorphism (SNP) detection selectivity. This method makes use of both DNA probe immobilized silica nanoparticles and optically amplifying light harvesting molecules. The recognition is accomplished by sequence-specific hybridization between the DNA probes on the silica ...

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  • Label-Free Single-Nucleotide Polymorphism

    2004-11-1 · Pack the column. 1. Wash the gel-filtration medium in a large plastic beaker with 5% ethanol. Let the medium settle and adjust the volume of liquid to yield a gel slurry concentration of 65% to 75%. The XK 16/100, 26/100, and 50/100 columns are 100 cm long and have inner diameters of 16, 26, and 50 mm, respectively.

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  • Preparation and extraction of insoluble (inclusion

    Figure Legend Snippet: Comparison between Pt and PEDOT:PSS electrodes – gel images. Images of the Rainbow Marker separated in an 11-mm-wide PHAST PAGE gel showing the equivalence of the separation of GE Healthcare’s Full Range Rainbow Recombinant Protein Molecular Weight Marker driven with PEDOT:PSS electrodes (left) and with Pt electrodes (right).

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