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anionic polyacrylamide lcms using method

  • Evaluation of the Performance of an LC-MS/MS

    Waters previously developed a method for the determination of anionic polar pesticides in various food commodities based upon extraction with the established Quick Polar Pesticides method (QuPPe) from the EURL SRM in Stuttgart and LC-MS/MS using the Anionic Polar Pesticide column.

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  • Evaluation of the Performance of an LC-MS/MS Method

    2021-3-29 · Evaluation of the Performance of an LC-MS/MS Method for the Determination of Anionic Polar Pesticide Residues in Crops and Foodstuffs Using an Interlaboratory Study : Waters Subject This application brief shows the successful evaluation of the performance of this method by interlaboratory study using ACQUITY UPLC systems and Xevo TQ-XS tandem quadrupole mass spectrometers.

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  • The use of LC-MS/MS and GC-MS methods for the ...

    2012-12-13 · High throughput LC-MS/MS quantification of anionic surfactant mixture: An LC-MS/MS method was developed for the quantification of the SAS-MES mixture in detergent formulations. A high throughput LC- MS/MS method is now under development for the quantification of complex mixtures of sulfated, sulfonated, and phosponated surfactants.

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  • A multi-family offline SPE LC-MS/MS analytical

    The final analytical method was validated and applied for the quantification of surfactants in 36 river water samples. Method limits of quantification (LQ), intra and inter days precision and trueness were evaluated. With LQ between 15 and 485 ng/L, and trueness over 80%, this method was suitable for monitoring surfactants in surface water.

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  • [PDF] Synthesis of a cationic polyacrylamide by a ...

    Synthesis of a cationic polyacrylamide by a photocatalytic surface-initiated method and evaluation of its flocculation and dewatering performance: nano-TiO2 as a photo initiator. In the face of complex water quality changes, the application of existing cationic polyacrylamide has been largely limited. In this study, a series of cationic ...

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  • Determination of the concentrations of

    Abstract. This paper presents a laboratory study on the effectiveness of the nitrogen digestion method to determine the polymer concentrations for 3 different kinds of polyacrylamide–type polymers including partially hydrolyzed polyacrylamides, sulfonated polyacrylamides, and cationic polyacrylamides.

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  • The Strategy of Surfactant Analysis by HPLC

    2016-2-16 · Highly Sensitive and Selective Method for the Determination of Anionic Surfactants Using Suppressed Conductivity Detection The Acclaim PolarAdvantage II (PA2) column provides excellent hydrolytic stability in both acidic and alkaline conditions, and is ideal for highly selective and sensitive analysis for anionic surfactants using

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  • Polyacrylamide degradation and its implications in ...

    2018-9-7 · The hydrolyzed form of polyacrylamide (HPAM), a co-polymer of acrylamide and acrylic acid, is the most widely used anionic PAM in oil and gas development as well as …

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  • Quantitative determination of multiple surfactants in ...

    In a second aspect, the present invention provides a method for detecting an anionic protein in a sample. This method comprises contacting said sample with a compound which has a formula selected from: in which R 1, R 2, R 7, and R 8 are substituted or unsubstituted alkyl. R 4, R 5, R 10, and R 11 are halogen. The product of step a) is ...

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  • Size Exclusion Chromatography in the Presence of ... -

    2020-12-5 · anionic surface, as depicted in Figure 1. Typically, SDS is used at significantly higher concentrations of 2% w/v for sample preparation for SDS-PAGE, but also with a reducing agent to cleave disulfide bonds within the protein molecule. The resulting saturated SDS-protein complex has, on average, 1.4 g SDS per gram of protein [1].

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  • Effect of Polyacrylamide on Improvement of Dredger

    This paper investigates an advanced treatment method using chemically additive polyacrylamide (PAM) on dredger fill combined with a vacuum preloading method via laboratory model tests. Anionic polyacrylamide (APAM, a PAM class based on ionic classification) exhibited better performance on accelerating water discharge, reducing the time for ...

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  • Anionic Polymer Flocculant & Polyacrylamide Powder ...

    2016-2-16 · Highly Sensitive and Selective Method for the Determination of Anionic Surfactants Using Suppressed Conductivity Detection The Acclaim PolarAdvantage II (PA2) column provides excellent hydrolytic stability in both acidic and alkaline conditions, and is ideal for highly selective and sensitive analysis for anionic surfactants using

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  • The Strategy of Surfactant Analysis by HPLC

    2008-11-1 · The electrokinetic property and electric repulsion between sample components and anionic groups on the polyacrylamide gel layer produce, trap, and concentrate anions within a few minutes at the interface of the cathodic side of the gel layer. This method …

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  • In situ fabrication of ionic polyacrylamide-based ...

    2021-3-31 · Surfactant chemistry has made a considerable impact on a number of household products including detergents, shampoos and toothpaste. Products are generally classified by the type of each hydrophilic substructure into anionic, cationic, amphoteric and non-ionic surfactants. Either anionic or non-ionic surfactants are typically used as synthetic detergents, however, to better elucidate the ...

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  • Simultaneous analysis of anionic, amphoteric and non

    2018-12-11 · The ionic polyacrylamide/graphene oxide (PAM/GO) hydrogel as an excellent methylene blue (MB) adsorbent was prepared by an improved one-step radical polymerization method using graphene oxide, acrylamide, and polar second monomers.

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  • Quantification of gel-separated proteins and their ...

    Phosphoproteins, isolated from total cell lysates using IMAC or by immunoprecipitation using Tyr(P) antibodies, were analyzed using this method, leading to identification of >400 proteins, several of which were found at higher levels in phosphoprotein fractions after pervanadate treatment.

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  • Evaluation of sample preparation methods for mass ...

    2018-8-25 · Sample preparation is a critical process for proteomic studies. Many efficient and reproducible sample preparation methods have been developed for mass spectrometry-based proteomic analysis of human and animal tissues or cells, but no attempt has been made to evaluate these protocols for plants. We here present an LC–MS/MS-based proteomics study of barley leaf aimed at …

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  • Detection of simultaneous production of kurstakin ...

    2021-5-10 · To enable thorough studies of lipopeptides, this paper describes a new method for purification and characterization of in-gel anionic lipopeptides. Specifically, lipopeptides attributed to the anti-staphylococcal activity of Bacillus mojavensis HF were separated using SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis) and ...

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  • Fabricating an anionic polyacrylamide (APAM) with an ...

    2016-2-17 · method is significantly lower than the MDL using an LC-MS method with a trimode column (in press), making IC-MS the preferred method for low-level quantification. Analysis of Commercial Ionic Liquids Three commercially available ionic liquids (dissolved in DI water at 2 mg/mL) were analyzed for impurities by the IC-MS method. Eight

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  • IC-MS Determination of Anionic Ionic Liquids,

    2001-8-4 · Using polyacrylamide (PAM) to reduce soil erosion in irrigated land has increased rapidly in recent years. A simple and reliable method to measure the PAM concentration in waters containing dissolved organic matter (DOM) is of great importance in assessing the fate and efficiency of PAM application. In this research, an analytical method to determine the PAM concentration of waters with ...

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  • Spectrophotometric Determination of Polyacrylamide

    2019-10-3 · The main purpose of the study was to examine lead(II) ion immobilization on the kaolinite surface and determine the effect of soil flocculant (macromolecular compound) on this process. For this reason, the adsorption measurements of anionic polyacrylamide and/or Pb(II) ions on the kaolinite surface were performed. Moreover, the impact of heavy metal on electrokinetic and stability properties ...

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  • Anionic polyacrylamide as a substance strengthening

    2021-3-16 · Simultaneous analysis of anionic, amphoteric and non-ionic surfactants using ultra-high speed LC-MS/MS Current surfactant monitoring methodologies tend to focus on a specific surfactant. Here, we have developed the simultaneous analysis method for typical anionic, amphoteric and non-ionic surfactant using LC-MS/MS.

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  • Simultaneous analysis of anionic, amphoteric and non

    2021-3-31 · Surfactant chemistry has made a considerable impact on a number of household products including detergents, shampoos and toothpaste. Products are generally classified by the type of each hydrophilic substructure into anionic, cationic, amphoteric and non-ionic surfactants. Either anionic or non-ionic surfactants are typically used as synthetic detergents, however, to better elucidate the ...

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  • Hyperfloc MG 655(anionic polyacrylamide) used for

    2008-11-1 · The electrokinetic property and electric repulsion between sample components and anionic groups on the polyacrylamide gel layer produce, trap, and concentrate anions within a few minutes at the interface of the cathodic side of the gel layer. This method …

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  • Simultaneous analysis of anionic, amphoteric and non

    2018-9-7 · The hydrolyzed form of polyacrylamide (HPAM), a co-polymer of acrylamide and acrylic acid, is the most widely used anionic PAM in oil and gas development as well as …

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  • In situ fabrication of ionic polyacrylamide-based ...

    1. A method for determining the presence or absence of an anionic protein and the presence or absence of a non-anionic protein in a sample, the method comprising: a) contacting the sample with a dye to form a labeled sample, wherein the dye has the following formula: in which X is wherein at most one of the H is replaced with wherein R 1 is hydrogen, alkyl or substituted alkyl; R 2 is hydrogen ...

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  • Fabricating an anionic polyacrylamide (APAM) with an ...

    2021-2-19 · Method Performance In our investigations, 100 µL injections on a 150 x 3.0 mm Allure Acrylamide column under the conditions given in Figure 2 proved to be the optimal choice for the analysis of acrylamide in drinking water by direct, large volume injection.

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  • LC-MS/MS Analysis of Acrylamide in Drinking Water

    2021-3-16 · Simultaneous analysis of anionic, amphoteric and non-ionic surfactants using ultra-high speed LC-MS/MS Current surfactant monitoring methodologies tend to focus on a specific surfactant. Here, we have developed the simultaneous analysis method for typical anionic, amphoteric and non-ionic surfactant using LC-MS/MS.

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  • Simultaneous analysis of anionic, amphoteric and non

    Graphene oxide (GO) have been reported as adsorbent materials, because its surface contains a large number of oxygen-containing groups, which provide masses of active sites. Nevertheless, it is difficult to separate GO from aqueous solution by conventional means after the end of the adsorption process. Therefore, ethylene diamine-reduced graphene oxide/anionic polyacrylamide (E-RGO/APAM), with ...

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  • Preparation of RGO and Anionic Polyacrylamide

    Potential Use of Polyacrylamide for Soil Erosion Control in Brazil Alexandre Marco da Silva (Corresponding author) ... including gypsiferous materials and anionic polyacrylamide (Cochrane et al., 2005). ... ha −1 y −1 for annual crops, 13.9 t ha y for pasture and 5.48 t ha−1 y−1 for coffee (the method used was Cesium-137). Soil erosion ...

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  • Potential Use of Polyacrylamide for Soil Erosion Control ...

    The polyacrylamide has a wide model range, the most common models are the anionic polyacrylamide, the cationic polyacrylamide and the nonionic polyacrylamide. ... Using the strong oxidation agent, or using the strong alkali, but this method must be considered with the consideration of the industrial production conditions in practical.

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  • The stability of the polyacrylamide flocculant under ...

    2012-7-23 · Acrylamide + Acrylic Acid + Caustic Soda Anionic polyacrylamide Note : with m = 0, the polyacrylamide is non-ionic NON IONIC AND ANIONIC POLYACRYLAMIDES Copolymers of acrylamide and acrylic acid 1.2. The Flocculants : 1.2.1. Characteristics : Organic flocculants differ …

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  • PREPARATION OF ORGANIC POLYMERS - SNF

    2013-8-23 · Shampoos contain various combinations of anionic, cationic, nonionic, and amphoteric surfactants, designed to influence the essential characteristics of the shampoo: shampoos to provide lather and cleansing. As shown in Figure 5, the individual oligomers in laureth sulfate are well resolved using the Acclaim Surfactant Plus column. FIGURE 5.

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  • High-Resolution and High-Throughput Surfactant

    2021-4-26 · An HPLC phase-switching method has been developed in an attempt to overcome the problem of an interfering anionic species (thought to be humic acids) that masks the presence of any linear alkylbenzene sulphonate surfactants in river water samples. This problem has arisen following the development of an HPLC method for the

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  • A Sheffield Hallam University thesis

    2018-11-8 · using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to the instruction of the manufacturer. A six-His tag was introduced at the C-terminal end of the open-reading frame of OATP2B1*3. Transfected cells were incubated for 24h at 37 C and then used for immunofluorescence, surface biotinylation, and transport assay. Immunofluorescence

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  • salvador erosion control using anionic polyacrylamide

    A Guide to Using Polyacrylamide (PAM) Polymers for Control of . erosion. PAM is also used for dust control on unpaved roads. Various forms of PAM exist, but the type used for erosion control is a large, negatively (anionic) charged molecule (1215 megagrams per mole) that is water soluble.

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  • The method of obtaining anionic polyacrylamide

    (57) Abstract: The invention relates to methods for anionic polyacrylamide method of hydrolysis. Describes how to obtain anionic polyacrylamide, including hydrolysis 10-50% aqueous gel of polyacrylamide water when heated carried out continuously and under pressure, characterized in that the hydrolysis is carried out in a period of 10-90 min at a temperature of 150-220 o C by filing the ...

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  • Preparation of RGO and Anionic Polyacrylamide

    Graphene oxide (GO) have been reported as adsorbent materials, because its surface contains a large number of oxygen-containing groups, which provide masses of active sites. Nevertheless, it is difficult to separate GO from aqueous solution by conventional means after the end of the adsorption process. Therefore, ethylene diamine-reduced graphene oxide/anionic polyacrylamide (E-RGO/APAM), with ...

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  • A Practical Approach on SDS PAGE for Separation of Protein

    2020-4-19 · using this method, due to the greater variability in the ratio of bound SDS. Keywords: SDS-PAGE, molecular biology, biotechnology, electrophoretic mobility 1. Introduction Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a technique for separating proteins based on their ability to move within an electrical current, which

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  • Green synthesis of fluorescent Ag-anionic

    The fluorescent Ag-anionic polyacrylamide (Ag-APAM) hybrid nanoparticles (NPs) gels solutions were synthesized by using an environmental-friendly APAM template without extra reductant and stabilizer. The APAM with stretched molecule chains trended to absorb and reduce Ag + to Ag 0 under the UV-light of 365 nm. Moreover, the network structure formed by APAM can protect Ag NPs from the ...

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  • Potential Use of Polyacrylamide for Soil Erosion Control ...

    Potential Use of Polyacrylamide for Soil Erosion Control in Brazil Alexandre Marco da Silva (Corresponding author) ... including gypsiferous materials and anionic polyacrylamide (Cochrane et al., 2005). ... ha −1 y −1 for annual crops, 13.9 t ha y for pasture and 5.48 t ha−1 y−1 for coffee (the method used was Cesium-137). Soil erosion ...

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  • Synthesis of a Cationic Polyacrylamide under UV

    2.3. Characterization of Copolymers. The intrinsic viscosities and the cationic degree (CD) of polymers were determined by using an Ubbelohde viscosity meter (Shanghai Shenyi Glass Instrumental Co. Ltd., China) at 30°C and colloid titration method, respectively [15, 18].In addition, the FT-IR spectra of polymers were conducted on a 550 Series II infrared spectrometer (Mettler Toledo ...

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  • Polyacrylamide Gel Electrophoresis (PAGE ...

    2018-10-20 · Electrophoresis through agarose or polyacrylamide gels is a standard method used to separate, identify and purify biopolymers, since both these gels are porous in nature.; Polyacrylamide gels are chemically cross-linked gels formed by the polymerization of acrylamide with a cross-linking agent, usually N,N’-methylenebisacrylamide.

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  • Derivatization and Sample Prep for (Small) Molecules

    2019-12-18 · • the method is demonstrated in this example using standards • results of plasma & urine samples are in agreement with the same samples analyzed by the standard method (GC/MS) References: Magera et al, Clinical Chem. 2000, 46 (11): 1804-1810 Schmedes et al, Clinical Chem. 2006, 52(4): 754-757 MS/MS of Derivatized MMA and Succinic Acid

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  • Improved LC/MS Methods for the Analysis of Anionic

    2018-6-15 · • This method could also be applied to additional metal-sensitive applications utilizing ion-pairing reverse phase and ion exchange chromatography. Conclusions. References. 1. J.J. Pesek, M.T. Matyska, S.M. Fischer. “Improvement of peak shape in aqueous normal phase analysis of anionic metabolites”, J Sep Sci, 34(24):3509-16, 2011. 2.

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  • Sinofloc Polyacrylamide with AMPS - Sinofloc Chemical

    For high brines conditions in fracturing, SINOFLOC team synthesized the anti-temperature and salt-resistant fracturing fluid thickener with acrylamide monomer and AMPS and by using free copolymerization of an aqueous solution. The optimum conditions of synthesis were determined by lab experiments. The results show that the fracturing fluid has excellent resistance to temperature and salt ...

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  • Anionic Surfactant SAFETY DATA SHEET Additive

    2017-8-16 · 1925 Anionic Surfactant Additive Revision Date: 27-Jun-2017 smoke when using this product. 9. PHYSICAL AND CHEMICAL PROPERTIES Information on basic physical and chemical properties Other information Density 9.00 lb/gal 10. STABILITY AND REACTIVITY Reactivity Stable under normal conditions. Chemical Stability Stable under normal conditions.

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  • Nanoparticles as Drilling Fluids Rheological Properties ...

    2019-8-24 · grown ZnO nanowires by chemical vapor deposition method. Table 1: Drilling fluid additives as received from Baroid- Halliburton Company. Additive Material Concentration Lb/Bbl Bentonite 15 EZ MUD (partially hydrolyzed polyacrylamide) 1 PAC RE (Poly Anionic Cellulose) 2.5 Magnesium Oxide (MgO) 0.5 Soda ash (Na 2 CO 3) 0.25 SALT (NaCl) 33

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  • PPARα-UGT axis activation represses intestinal FXR

    Colitis was induced using the method described before with little modification52. In brief, colitis was induced by administration of 2.5% (w/v) DSS in drinking water for 7 days and a 14-day washout with drinking water for 3 cycles. Faecal and urine excretion of bile acid was determined in stools collected over 24 h in metabolic cages.

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  • LC/MS method of analyzing high molecular weight

    2005-1-21 · A method of determining the presence of an antibody degradation product in an antibody sample, said method comprising: a. performing RP-HPLC on said antibody sample under conditions wherein the HPLC column is heated to a temperature of from about 50° C. to about 90° C.; and wherein the mobile phase of said reversed-phase HPLC comprises a ...

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  • A pH-correctable, DNA-based fluorescent reporter for ...

    2018-12-10 · The DNA-based, ratiometric fluorescent reporter CalipHluor enables quantitative imaging of pH and calcium in acidic organelles with single-organelle resolution. The probe was used to identify a ...

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  • Proteomic analysis of a rat pancreatic stellate cell line ...

    2011-9-25 · Proteomic analysis of a rat pancreatic stellate cell line using liquid chromatography tandem mass spectrometry (LC-MS/MS). ... (lithium dodecyl sulfate) sample buffer (NP0008), NuPAGE 4-12% Bis-Tris polyacrylamide gels (NP0335), SimplyBlue Coomassie stain (LC0665), and MES-SDS electrophoresis buffer (NP002) were from Invitrogen (Carlsbad, CA ...

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  • Post-Assembly Functionalization of Supramolecular ...

    2021-3-12 · Post-assembly functionalization of supramolecular nanostructures has the potential to expand the range of their applications. We report here the use of the chemoselective native chemical ligation (NCL) reaction to functionalize self-assembled peptide amphiphile (PA) nanofibers. This strategy can be used to incorporate specific bioactivity on the nanofibers, and as a model, we demonstrate ...

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  • An improved method for the detection of anionic ...

    An improved method for the detection of anionic polymorphic proteins in human saliva on polyacrylamide slab ge ls. Friedman RD, Allushuski R. Analytical Biochemistry, 01 May 1975, 65(1-2): 561-566 DOI: 10.1016/0003-2697(75)90546-1 PMID: 236699. Share this article Share with email Share with ...

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  • Linear anionic polyacrylamide as an effective post-fire ...

    Controlling erosion, reestablishing vegetation, and overcoming the negative effects of hydrophobic soils has long been a challenge following catastrophic wildfire on forested lands and rangelands. A three-year controlled study was recently completed to compare polyacrylamide soil treatment to the traditional cover method using agricultural straw on high severity burned soils of the Red Bull ...

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  • Using polyacrylamide with sprinkler irrigation to

    Center-pivot irrigation systems often apply water at rates greater than the soil infiltration rate. Applying high molecular weight, water-soluble, anionic polyacrylamide (PAM) to the soil can improve infiltration and reduce soil erosion The objective of this study was to determine whether single and multiple PAM applications with sprinkler irrigation improved infiltration under field conditions.

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  • The impact of anionic polyacrylamide (APAM) on ...

    2017-2-16 · With the purpose of improving the ultrafiltration (UF) efficiency, anionic polyacrylamide (APAM) has been used as a coagulant aid in the flocculation-UF process. In this study, the impact of APAM on UF efficiency has been investigated with regard to …

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  • A Guide to Using Polyacrylamide (PAM) Polymers for

    2010-12-15 · A Guide to Using Polyacrylamide (PAM) Polymers for Control of Irrigation Run-off on the Central Coast . Michael Cahn, Irrigation and Water Resources Advisor, Monterey County . Introduction . Federal and state water quality regulations require that growers implement management practices to minimize impairments to surface and ground water quality.

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  • A rapid and green method for the removal of anionic

    2019-6-13 · A novel and green adsorbent based on sulfobetaine-modified magnetic Fe 3 O 4 nanoparticles (SBMNPs) was successfully synthesized via a convenient one-step chemical coprecipitation method and applied to the removal of the anionic dyes methyl blue (MB) and amaranth (AM) from aqueous solution. The morphology and properties of the SBMNPs were characterized using …

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  • The principle and method of polyacrylamide gel ...

    Polymerized acrylamide (polyacrylamide) forms a mesh-like matrix suitable for the separation of proteins of typical size. The strength of the gel allows easy handling. Polyacrylamide gel electrophoresis of SDS-treated proteins allows researchers to separate proteins based on their length in an easy, inexpensive, and relatively accurate manner.

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  • Passive sampling of anionic pesticides using the

    DGT passive samplers using Oasis® HLB or Oasis® MAX sorbent were developed for anionic pesticides sampling. They were tested using four model compounds (i.e. bentazon, chlorsulfuron, ioxynil and mecoprop). Polyacrylamide diffusive gel was found to be more suitable than agarose gel for most anionic pesticides sampling. An elution procedure was optimized and diffusion coefficients were ...

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  • Polyacrylamide-Phytic Acid-Polydopamine

    2017-8-11 · Semi-empirical PM6-D3H4 method was employed to further figure out the adsorption mechanism between the PAAM/PA/PDA hydrogel and dye molecules by using Mopac2012 program since weak interactions in ...

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  • A review of analytical methods for the determination of ...

    2005-4-12 · This method has a minimum detection limit of 0.002 ng/mL for TCE from a 50 mL water sample (Munch and Hautman, 1995). A minimum detection limit (MDL) of 0.02 ng mL TCE was obtained with the GC-MS procedure described in USEPA Method 524.2 (Munch, 1995). Another GC-MS study was performed using USEPA Method 524.2, in which drinking water was analyzed

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