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5 cationic polyacrylamide gel recipe making process

  • Effect of cationic polyacrylamide on the

    2015-6-1 · Nanocellulose thin films were prepared by engineering the cellulose fibre flocculation with a series of high molecular weight cationic polyacrylamide (CPAM) using a papermaking-like process. The nanocellulose film properties and process efficiency were strongly affected by the nanofibres floc properties controlled with CPAM charge …

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  • Cationic polyacrylamide(high degree of ion)

    Cationic polyacrylamide(high degree of ion) used for sludge dewatering: 1.Solid content:89% 2.Degree of ion:5-65%(adjustable) 3.Molecular weight:6-15million 5.Disolving time:60mins. CHINAFLOC——Top ten in China!

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  • US3503946A - Process for the manufacture of

    A reaction mixture of 120 g. (1.7 mol) of polyacrylamide of 4,000,000 molecular weight, 700 ml. (5.6 mol) of 3-(dimethylamino)propylamine and 700 ml. of ethylene glycol in a reaction vessel fitted...

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  • Polyacrylamide (PAM) - DXD

    Industrial sewage dewatering treatment, cationic polyacrylamide, flocculant. Main Production: 1. Oilfield Chemicals, Oilfield chemical is a general germ,The oilfield chemicals we can supply include anionic polyacrylamide used for oil drilling mud additives , drag reduction agent and EOR(enhanced oil recovery)agent . 2.

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  • cationic polyacrylamide flocculant , China

    2019-2-5 · polyacrylamide around gel. 19. Hold two pieces of dry 46 × 57–cm blotting paper together as one piece. Beginning at one end of gel and working slowly towards the other, lay paper on top of gel. Take care to prevent air bubbles from forming between paper and gel. 20. Peel blotting paper up; gel should come off plate with it. Gradually …

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  • Denaturing Polyacrylamide Gel Electrophoresis

    Alibaba.com offers 4,496 textile chemical cationic polyacrylamide products. A wide variety of textile chemical cationic polyacrylamide options are available to you, such as classification, usage, and type.

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  • Introduction to Polyacrylamide Gels | LSR | Bio-Rad

    On the second day, the ingredients of the stacking gel are mixed, except for SDS and TEMED, and the mixture is degassed. The isobutanol layer on the separation gel is removed with a syringe and needle, and the surface is rinsed several times with distilled water. A small layer of water is left on the gel.

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  • Preparation of cationic polyacrylamide by aqueous

    monomers concentration 8~15%, PEG 20000 concentration 15~25%, and PDMC concentration 0.5~1.5%. Finally, the for-mation process of copolymer particles was investigated by optical microscope. Keywords: polymer synthesis, aqueous two-phase polymerization, cationic polyacrylamide, dimethylaminoethyl methacry-late methyl …

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  • Cationic polyacrylamide. Hoá chất

    Application of SPECFLOC C1492. One of the largest uses for polyacrylamide is to flocculate solids in a liquid. This process applies to water treatment, and processes like paper making and screen printing. Polyacrylamide can be supplied in a powder or liquid form, with the liquid form being subcategorized as solution and emulsion polymer.

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  • Polyacrylamide - an overview | ScienceDirect

    Polyacrylamide (12.5 %) gel slab electrophoresis in 0.1% sodium dodecyl sulphate was carried out as described by Laemmli (9). Analytical IEF in the pH range 2.5–7.0 was performed on 5.0 % acrylamide gel slab using a Multiphor II electrophoresis system (Pharmacia Biotech) following the manufacturer's instructions. Non-denaturing.

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  • White Water Cleaning Chemicals Cationic

    Polyacrylamide (IUPAC poly (2-propenamide) or poly (1-carbamoylethylene), abbreviated as PAM) is a polymer (-CH2CHCONH2-) formed from acrylamide subunits. It is highly water-absorbent, forming a soft gel when hydrated, used in such applications as polyacrylamide gel electrophoresis, and can also be called ghost crystals when cross …

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  • A Guide to Polyacrylamide Gel Electrophoresis and

    2018-3-5 · into two sections (a large-pore stacking gel on top of a small-pore resolving gel, Figure 2.2) and different buffers in the gels and electrode solutions (Wheeler et al. 2004) In gel electrophoresis, proteins do not all enter the gel matrix at the same time. Samples are loaded into wells, and the proteins that are closer to the gel

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  • Polyacrylamide precast gels for electrophoresis,

    The analysis of nucleic acids mostly depends on either the agarose electrophoresis separation process or the precast polyacrylamide gel electrophoresis separation process. As nucleic acids present strong negative charges in the neutral buffer solution while the mobility thereof depends on the molecular sieve effect of supportive matrix gels ...

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  • The principle and method of polyacrylamide gel ...

    Polymerized acrylamide (polyacrylamide) forms a mesh-like matrix suitable for the separation of proteins of typical size. The strength of the gel allows easy handling. Polyacrylamide gel electrophoresis of SDS-treated proteins allows researchers to separate proteins based on their length in an easy, inexpensive, and relatively accurate manner.

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  • WO2007121981A1 - Cationic polysaccharide, its

    The present invention relates to polysaccharide, preferably starch, of high purity having cationic degree of substitution over 0.50 and bound nitrogen index after cationisation reaction stage over 0.75, and to preparation thereof as well as to its use in making of paper or paperboard.

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  • Best Practices Guidance for the Use of Anionic ...

    2018-4-4 · 11/15/2017 5 What is Anionic Polyacrylamide? • One of the most common polymer flocculants on the market • Common uses of PAM as a flocculant: reduction of sediment and nutrient loads to natural lakes and ponds wastewater and drinking water treatment clarification of effluents in industries like pulp & paper, aquaculture • Also …

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  • SDS-PAGE Gel - CSHL P

    SDS-PAGE Gel. 1. Prepare the separation gel (10%). Mix in the following order: After adding TEMED and APS to the SDS-PAGE separation gel solution, the gel will polymerize quickly, so add these two reagents when ready to pour. 2. Pour gel, leaving ∼2 cm below the bottom of the comb for the stacking gel. Make sure to remove bubbles.

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  • Cationic polyacrylamide. Top Hóa chất bán lẻ,

    Application of SPECFLOC C1492. One of the largest uses for polyacrylamide is to flocculate solids in a liquid. This process applies to water treatment, and processes like paper making and screen printing. Polyacrylamide can be supplied in a powder or liquid form, with the liquid form being subcategorized as solution and emulsion polymer.

    Get Price
  • White Water Cleaning Chemicals Cationic

    Polyacrylamide (IUPAC poly (2-propenamide) or poly (1-carbamoylethylene), abbreviated as PAM) is a polymer (-CH2CHCONH2-) formed from acrylamide subunits. It is highly water-absorbent, forming a soft gel when hydrated, used in such applications as polyacrylamide gel electrophoresis, and can also be called ghost crystals when cross …

    Get Price
  • The principle and method of polyacrylamide gel ...

    Polymerized acrylamide (polyacrylamide) forms a mesh-like matrix suitable for the separation of proteins of typical size. The strength of the gel allows easy handling. Polyacrylamide gel electrophoresis of SDS-treated proteins allows researchers to separate proteins based on their length in an easy, inexpensive, and relatively accurate manner.

    Get Price
  • Cas 9003-05-8 Flocculant Cationic

    High quality Cas 9003-05-8 Flocculant Cationic Polyacrylamide Powder from China, China's leading Cas 9003-05-8 product, with strict quality control Flocculant Cationic Polyacrylamide Powder factories, producing high quality 100 Mesh Cationic Polyacrylamide Powder products.

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  • Cas 9003-05-8,Polyacrylamide | lookchem

    Protein polyacrylamide gel electrophoresis: (1)The basic principle of SDS denaturing in-continuous polyacrylamide gel electrophoresis is based on differences in the molecular weight of the protein, SDS is an anionic surfactant, capable of binding with the hydrophobic portion of the protein thus making the protein bring a large number of …

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  • Polyelectrolyte (Polyacrylamide Type) | DUBI CHEM

    2021-1-8 · From Fig. 5, the equilibrium swelling values for hydrogels were found as 8.5, 12.72, 19.51, and 25.52 g water g dry gel −1 for alginate concentration of 0, 0.25, 0.5, and 1 g, respectively. As a result, the swelling contents of alginate-based hydrogels are higher than that of polyacrylamide hydrogel (alginate concentration is zero).

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  • Absorption and adsorption studies of

    2016-8-9 · A 20-mer oligonucleotide is typically recovered in a 80 % yieldafter 3 hours of rotary shaking thereby making this technique comparableto electroelution. Since elution isa diffusion-controlled process, more buffer will aid in elution efficiency.Also, note that longer oligonucleotides will take longer to diffuse fromthe gel.

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  • BASIC PROTOCOL: PURIFICATION OF

    2017-5-4 · Polyacrylamide (PAM) is a long chain, linear, water soluble polymeric substance formed from acrylamide (C 3 H 5 NO) subunits, has a high molecular weight of typically from few 1000 to 20 × 10 6 g/mol, and has a very high viscosity in aqueous solutions, depending on the concentration and the degree of polymerization (Sojka et …

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  • Biodegradation of Polyacrylamide and Its

    SDS-PAGE Gel. 1. Prepare the separation gel (10%). Mix in the following order: After adding TEMED and APS to the SDS-PAGE separation gel solution, the gel will polymerize quickly, so add these two reagents when ready to pour. 2. Pour gel, leaving ∼2 cm below the bottom of the comb for the stacking gel. Make sure to remove bubbles.

    Get Price
  • Preparation of cationic polyacrylamide by aqueous

    monomers concentration 8~15%, PEG 20000 concentration 15~25%, and PDMC concentration 0.5~1.5%. Finally, the for-mation process of copolymer particles was investigated by optical microscope. Keywords: polymer synthesis, aqueous two-phase polymerization, cationic polyacrylamide, dimethylaminoethyl methacry-late methyl …

    Get Price
  • Cationic polyacrylamide. Hoá chất

    2021-5-9 · Cationic Polyacrylamide Flocculant Powder - Sinofloc Supplier. The polyacrylamide is a synthetic cationic polymerization flocculants and so mainly related to chain length and the charge of the dewatering performance of polyacrylamide excessive dosage, the positive charge on the ion polyacrylamide adsorption, due to electrostatic …

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  • polymer flocculant cationic polimer

    Cationic polyacrylamide emulsion used for wastewater. The cationic polyacrylamid emulsion is moisture absorbent, thus to protect from damp and moist for conservation. It should be stored in a dry and ventilate storehouse, and not be exposed to air and sunshine. tag:cationic polyacrylamide emulsion ,cationic flocculant,wastewater …

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  • cationic polymer polyacrylamide flocculant

    Non Denaturing Polyacrylamide Gel Recipe. Bryont Rugs and Livings June 5, 2018. ... Polyacrylamide gel electropses a denaturing in tbe 7 m a non denaturing polyacrylamide 12 gel electropsis of dna native gel electropsis you polyacrylamide gel electropses a denaturing in tbe 7 m. Share.

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  • Non Denaturing Polyacrylamide Gel Recipe |

    affected the gel strength and the gel structure shift on time-dependent at low content. The optimized rheological modifier was obtained in present work which contained 3.5% magnesium oxide, 0.75% sodium polyacrylate, 0.5% alpha sodium naphthol sulfonate, and 0.05% cationic polyacrylamide in the attapulgite clay. Introduction

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  • Preparation and Rheological Properties of Attapulgite

    A precast acrylamide electrophoresis gel is disclosed, which includes a gel buffer solution containing either Tris and glycine or Tris, glycine and any one or more than two of ampholytes, and a monobasic acid, titrated to a specified pH range. With the gels constituted as stated earlier, proteins can be successfully subjected to electrophoresis …

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  • US6726821B1 - Polyacrylamide precast gels for ...

    2019-12-31 · 2 Polyacrylamide Emulsions Handbook Storage and handling 4. Storage and handling of emulsions : basic principles zzzEmulsions must be stored inside a building at a constant temperature between 5°C and 30°C. zzDuring the storage and handling, the

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  • Polyacrylamide Handbook - SNF

    The present invention relates to polysaccharide, preferably starch, of high purity having cationic degree of substitution over 0.50 and bound nitrogen index after cationisation reaction stage over 0.75, and to preparation thereof as well as to its use in making of paper or paperboard.

    Get Price
  • WO2007121981A1 - Cationic polysaccharide, its

    2019-4-9 · In recent years, template polymerization technology has been used to try to exert some degrees of control over the distribution of cationic units in CPAMs [].Like a genetic translation process, a low molecular weight polymer acts as an anionic template which can adsorb cationic monomers through electrostatic forces to realize an ordered arrangement of cationic …

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  • Cationic polyacrylamide. Top Hóa chất bán lẻ,

    Application of SPECFLOC C1492. One of the largest uses for polyacrylamide is to flocculate solids in a liquid. This process applies to water treatment, and processes like paper making and screen printing. Polyacrylamide can be supplied in a powder or liquid form, with the liquid form being subcategorized as solution and emulsion polymer.

    Get Price
  • cationic polyacrylamide powder and emulsion in

    2021-7-7 · Cationic Polyacrylamide Flocculant Powder - Sinofloc Supplier. The polyacrylamide is a synthetic cationic polymerization flocculants and so mainly related to chain length and the charge of the dewatering performance of polyacrylamide excessive dosage, the positive charge on the ion polyacrylamide adsorption, due to electrostatic …

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  • Polyacrylamide Emulsions Handbook - SNF

    2012-7-22 · Polyacrylamide Emulsions Handbook 2-2 Storage and handling of emulsions : basic principles Emulsions must be stored inside a building at a constant temperature between 5°C and 30°C. During the storage and handling, the emulsion must not be contaminated by water. Emulsions must not be in a situation where the surface can dry …

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  • Polyacrylamide - Hyperleap

    It is produced industrially as a precursor to polyacrylamides, which find many uses as water-soluble thickeners and flocculation agents. Aquamid is the trade name for a specific formulation of 97.5% water for injection and 2.5% cross-linked polyacrylamide. N,N-Methylenebisacrylamide' (MBAm or MBAA) is a cross-linking agent used during the formation of polymers such as polyacrylamide.

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  • Bluwat Cationic Polyacrylamide Flocculant

    High quality Bluwat Cationic Polyacrylamide Flocculant Flocking Agent Water Treatment from China, China's leading cationic polyelectrolyte flocculants product, with strict quality control industrial water treatment chemicals factories, producing high quality industrial water treatment chemicals products.

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  • machine for emulsion nonionic polyacrylamide in

    2021-7-8 · Saudi Arabia Polyacrylamide Market by Type, Opportunities. Saudi Arabia Polyacrylamide Market by Type (Anionic Polyacrylamide, Nonionic Polyacrylamide, Cationic Polyacrylamide and Others), By Form (Powder, Emulsion, Gel and Others), By Application (Wastewater Treatment, Oil & Gas Extraction, Mining, Paints & Coatings and …

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  • BASIC PROTOCOL: PURIFICATION OF

    2016-8-9 · A 20-mer oligonucleotide is typically recovered in a 80 % yieldafter 3 hours of rotary shaking thereby making this technique comparableto electroelution. Since elution isa diffusion-controlled process, more buffer will aid in elution efficiency.Also, note that longer oligonucleotides will take longer to diffuse fromthe gel.

    Get Price
  • Cationic Magnetite Nanoparticles for Increasing

    2020-5-27 · incubated for fixed periods (0, 0.5, 1, 2, or 3 h), with the subsequent addition of 100 mM PBS (pH = 7.4) (4 L), for sedimentation NPs, and centrifugation at 14,000 g for 5 min. 2.6. Evaluation of RNA Hybridization Level Using PAGE The supernatant obtained from centrifugation was analyzed using native polyacrylamide gel electrophoresis …

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  • Biodegradation of Polyacrylamide and Its

    2017-5-4 · Polyacrylamide (PAM) is a long chain, linear, water soluble polymeric substance formed from acrylamide (C 3 H 5 NO) subunits, has a high molecular weight of typically from few 1000 to 20 × 10 6 g/mol, and has a very high viscosity in aqueous solutions, depending on the concentration and the degree of polymerization (Sojka et …

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  • CHAPS Detergent: Protocols and Frequently

    A nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents. Commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without …

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  • Cationic polyacrylamide. Top Hóa chất bán lẻ,

    Application of SPECFLOC C1492. One of the largest uses for polyacrylamide is to flocculate solids in a liquid. This process applies to water treatment, and processes like paper making and screen printing. Polyacrylamide can be supplied in a powder or liquid form, with the liquid form being subcategorized as solution and emulsion polymer.

    Get Price
  • polyacrylamide polymer flocculant cationic

    2021-5-20 · Cationic Polyacrylamide Flocculant Powder - Sinofloc Supplier. The polyacrylamide is a synthetic cationic polymerization flocculants and so mainly related to chain length and the charge of the dewatering performance of polyacrylamide excessive dosage, the positive charge on the ion polyacrylamide adsorption, due to electrostatic …

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  • Bluwat Cationic Polyacrylamide Flocculant

    High quality Bluwat Cationic Polyacrylamide Flocculant Flocking Agent Water Treatment from China, China's leading cationic polyelectrolyte flocculants product, with strict quality control industrial water treatment chemicals factories, producing high quality industrial water treatment chemicals products.

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  • US6726821B1 - Polyacrylamide precast gels for ...

    A precast acrylamide electrophoresis gel is disclosed, which includes a gel buffer solution containing either Tris and glycine or Tris, glycine and any one or more than two of ampholytes, and a monobasic acid, titrated to a specified pH range. With the gels constituted as stated earlier, proteins can be successfully subjected to electrophoresis …

    Get Price
  • Cationic Polymer Water Treatment

    Cationic Polymer Water Treatment Polyacrylamide, US 1 - 1.3 Kilogram, Chemical Auxiliary Agent, polymer, nalcolyte 8103.Source from Yixing Bluwat Chemicals Co., Ltd. on …

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  • Blue Native Polyacrylamide Gel Electrophoresis (BN

    Blue native polyacrylamide gel elec- trophoresis (BN-PAGE) is a separation method with a higher resolution than gel filtration or sucrose density ultra- centrifugation that can be used to analyze abundant, stable MPCs from 10 kD to 10 MD.

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  • Nonionic Polyacrylamide Flocculant - Sinoloc

    2020-5-27 · incubated for fixed periods (0, 0.5, 1, 2, or 3 h), with the subsequent addition of 100 mM PBS (pH = 7.4) (4 L), for sedimentation NPs, and centrifugation at 14,000 g for 5 min. 2.6. Evaluation of RNA Hybridization Level Using PAGE The supernatant obtained from centrifugation was analyzed using native polyacrylamide gel electrophoresis …

    Get Price
  • Cationic Magnetite Nanoparticles for Increasing

    2018-10-20 · Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility. The most commonly used form of polyacrylamide gel electrophoresis …

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  • Polyacrylamide Gel Electrophoresis (PAGE ...

    Polyacrylamide, Dispersing Agent, Scale Inhibition manufacturer supplier in China, offering Polyacrylamide (PAM) Water Treatment Anionic Cationic Nonionic Dispersant, Home Water 1000liters 2000liters Water Softener, 5000liters/Hour Easy to Clean Horizontal Ultrafiltration Water Purifier and so on.

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  • Polyacrylamide Emulsions Handbook - SNF

    2012-7-22 · Polyacrylamide Emulsions Handbook 2-2 Storage and handling of emulsions : basic principles Emulsions must be stored inside a building at a constant temperature between 5°C and 30°C. During the storage and handling, the emulsion must not be contaminated by water. Emulsions must not be in a situation where the surface can dry …

    Get Price
  • Biodegradation of Polyacrylamide and Its

    2017-5-4 · Polyacrylamide (PAM) is a long chain, linear, water soluble polymeric substance formed from acrylamide (C 3 H 5 NO) subunits, has a high molecular weight of typically from few 1000 to 20 × 10 6 g/mol, and has a very high viscosity in aqueous solutions, depending on the concentration and the degree of polymerization (Sojka et …

    Get Price
  • machine for emulsion nonionic polyacrylamide in

    2021-7-8 · Saudi Arabia Polyacrylamide Market by Type, Opportunities. Saudi Arabia Polyacrylamide Market by Type (Anionic Polyacrylamide, Nonionic Polyacrylamide, Cationic Polyacrylamide and Others), By Form (Powder, Emulsion, Gel and Others), By Application (Wastewater Treatment, Oil & Gas Extraction, Mining, Paints & Coatings and …

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  • Paper Chemical - China Polyacrylamide, AKD

    Paper Making Chemical Deinking Agent Used as Bleaching Agent and Surface Sizing Agent. FOB Price: 1300 - 1500 Ton. Min. Order: 1 Ton. We are manufacturing facotry and exporter of deinking agent and paper making chemicals in China, we are located in Shang hai,very near to Shanghai Seaport and Airport.

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  • Blufloc A6518 Anionic Polyacrylamide High

    Iran Stone Cutting Wastewater Anionic Polyacrylamide Blu Floc Polyacrylamide 100% Pure. Chemical Name:Anionic Polyacrylamide. Trade Name:Blufloc AA5415. Molecular Weight:12-16Million. Contact Now.

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  • Anionic Polyacrylamide factory, Buy good quality

    99% Purity Chemical Polymers Polyacrylamide CAS NO. 9003-05-8 9003-05-8 Suppliers,provide 99% Purity Chemical Polymers Polyacrylamide CAS NO. 9003-05-8 9003-05-8 product and the products related with China (Mainland) 99% Purity Chemical Polymers Polyacrylamide CAS NO. 9003-05-8 9003-05-8 Hebei Bosang International …

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  • China Flocculant for Mineral Separation

    Polyacrylamide, Dispersing Agent, Scale Inhibition manufacturer supplier in China, offering Polyacrylamide (PAM) Water Treatment Anionic Cationic Nonionic Dispersant, Home Water 1000liters 2000liters Water Softener, 5000liters/Hour Easy to Clean Horizontal Ultrafiltration Water Purifier and so on.

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  • 99% Purity Chemical Polymers Polyacrylamide

    Sludge Dewatering Polymer, US 1.7 - 3.5 Kilogram, Chemical Auxiliary Agent, 9003-05-8, flocculant.Source from Yixing Bluwat Chemicals Co., Ltd. on Alibaba.com.

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