Enhanced production of recombinant proteins in ...
2020-5-26 · The PCR condition was: 95 °C for 30 s and 45 cycles at 95 °C for 15 s, 62 °C for 30 s, 72 °C for 20 s. The EGFP transcription level of pXMJ19-EGFP with a monocistronic P tac was defined as 1. Every sample was measured across at least three biological …
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